Frap Question

Started by wkawk2416
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wkawk2416

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What is the % recovery and lateral mobility for large multi-subunit integral membrane protein that is not anchored to the cytoskeleton?

a. High % recovery with fast mobility
b. Low % recovery with fast mobility
c. High % recovery with slow mobility
d. Low % recovery with low mobility

Originally posted by someone, but found in my word document.
 
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High percent recovery, but at low mobility or high is the question...

If its not anchored that means it will move throughout the membrane, FRAP = flourensence recovery after photobleaching...so if its moving the laser will catch it at high percent recovery...just i have no idea if its low mobility or high...someone HELP!!!!
 
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yea its A, its got to be....more mobility = faster and high percentage!!!

THIS QUESTION ISNT THAT BAD actually...
 
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i don't know about cholestrol being anchored, but it decreases fluidity of PM.

Also, double bond (kinks? kiks? kirks? whatever) , and short phospholipid will also increase the fluidity.
 
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but doesnt FRAP have to do with mobility of some substance on the membrane...i dont know if it has to do with the membrane itself being fluid or not...
 
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