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how do i dilute this: 10 mg/ml to 10 microgram/ml
Thanks
Thanks
how do i dilute this: 10 mg/ml to 10 microgram/ml
Thanks
let me rephrase:
a stock solution of 1 ml contain 10 mg of DNA.. how to i dilute it to become 10 microgram of DNA in 1 ml?
I think this question is impossible.
M1V1=M2V2
you start with 10mg in 1mL and it changes into 10ug in 1mL.
if the molarity changes, the volume has to change but the volume remains
the same so I blieve this question is impossible Unless I am mistaken.
let me rephrase:
a stock solution of 1 ml contain 10 mg of DNA.. how to i dilute it to become 10 microgram of DNA in 1 ml?
That's the right way.
In this case, M1 = 10 mg/ml, M2 = 10 ug/ml = 10 x 10^-3 mg/ml, V1 = 1 ml
From M1V1 = M2V2
V2 = 1000 ml
So, what you'll do is pour that 1 ml solution to a 1L beaker, rinse the stock container to get all DNA in. And then add water to the 1000 ml mark.
That's the right way.
In this case, M1 = 10 mg/ml, M2 = 10 ug/ml = 10 x 10^-3 mg/ml, V1 = 1 ml
From M1V1 = M2V2
V2 = 1000 ml
So, what you'll do is pour that 1 ml solution to a 1L beaker, rinse the stock container to get all DNA in. And then add water to the 1000 ml mark.
he needs to end up with 1 ml
but he said
you start with 1 ml and end with 1ml.
I don't know where you found 1000ml for V2?
the question is saying, "keep the volume the same, but dilute 1000 times).........so just take 1/1000 out (.001 ml of 10 mg -- this will make the 10 mg 1000 less ====== 10 ug) and put 999 parts (.999 ml) water to complete dilution....does it make sense????
btw u=micro
That's the Chem lab method. And may not apply in a small sample needing great dilution for a Bio lab here.
thefifthbeatle's approach is more practical.
lol...i had to do that very dilution in my lab today...
he needs to end up with 1 ml
says who? I didn't get that from the first post. maybe I missed something.