G Chem

[151AND8TH]

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how do i dilute this: 10 mg/ml to 10 microgram/ml
Thanks

 

[thefifthbeatle]

how do i dilute this: 10 mg/ml to 10 microgram/ml
Thanks

Well, There are 1000 ug in a mg


But, i've never seen densities being diluted

 

[151AND8TH]

let me rephrase:

a stock solution of 1 ml contain 10 mg of DNA.. how to i dilute it to become 10 microgram of DNA in 1 ml?

 

[joonkimdds]

let me rephrase:

a stock solution of 1 ml contain 10 mg of DNA.. how to i dilute it to become 10 microgram of DNA in 1 ml?

I think this question is impossible.
M1V1=M2V2
you start with 10mg in 1mL and it changes into 10ug in 1mL.
if the molarity changes, the volume has to change but the volume remains
the same so I blieve this question is impossible Unless I am mistaken.

 

[laundry]

lol...i had to do that very dilution in my lab today...

 

[tresbon]

I think this question is impossible.
M1V1=M2V2
you start with 10mg in 1mL and it changes into 10ug in 1mL.
if the molarity changes, the volume has to change but the volume remains
the same so I blieve this question is impossible Unless I am mistaken.

That's the right way.

In this case, M1 = 10 mg/ml, M2 = 10 ug/ml = 10 x 10^-3 mg/ml, V1 = 1 ml

From M1V1 = M2V2

V2 = 1000 ml

So, what you'll do is pour that 1 ml solution to a 1L beaker, rinse the stock container to get all DNA in. And then add water to the 1000 ml mark.

 

[thefifthbeatle]

let me rephrase:

a stock solution of 1 ml contain 10 mg of DNA.. how to i dilute it to become 10 microgram of DNA in 1 ml?

Nowhere NEAR impossible:

Take 1 ul from the 1 ml 10 mg of DNA and dilute it with .999 of a ml of H2O
or

the question is saying, "keep the volume the same, but dilute 1000 times).........so just take 1/1000 out (.001 ml of 10 mg -- this will make the 10 mg 1000 less ====== 10 ug) and put 999 parts (.999 ml) water to complete dilution....does it make sense????

btw u=micro

 

[thefifthbeatle]

That's the right way.

In this case, M1 = 10 mg/ml, M2 = 10 ug/ml = 10 x 10^-3 mg/ml, V1 = 1 ml

From M1V1 = M2V2

V2 = 1000 ml

So, what you'll do is pour that 1 ml solution to a 1L beaker, rinse the stock container to get all DNA in. And then add water to the 1000 ml mark.

he needs to end up with 1 ml

 

[joonkimdds]

That's the right way.

In this case, M1 = 10 mg/ml, M2 = 10 ug/ml = 10 x 10^-3 mg/ml, V1 = 1 ml

From M1V1 = M2V2

V2 = 1000 ml

So, what you'll do is pour that 1 ml solution to a 1L beaker, rinse the stock container to get all DNA in. And then add water to the 1000 ml mark.

but he said
you start with 1 ml and end with 1ml.

 

[tresbon]

he needs to end up with 1 ml

Then, stir the 1 L beaker well and take 1 ml out from there.

But, I suppose your approach is better, although great tool and hand skills are needed here.🙂

 

[tresbon]

but he said
you start with 1 ml and end with 1ml.
I don't know where you found 1000ml for V2?

That's the Chem lab method. And may not apply in a small sample needing great dilution for a Bio lab here.

thefifthbeatle's approach is more practical.👍

 

[joonkimdds]

the question is saying, "keep the volume the same, but dilute 1000 times).........so just take 1/1000 out (.001 ml of 10 mg -- this will make the 10 mg 1000 less ====== 10 ug) and put 999 parts (.999 ml) water to complete dilution....does it make sense????

btw u=micro

HEy! that's cheating!

 

[joonkimdds]

That's the Chem lab method. And may not apply in a small sample needing great dilution for a Bio lab here.

thefifthbeatle's approach is more practical.👍

the topic said Gchem so bio method is cheating 😛
i win~😛
JoonKimDDS = 👍 😀

 

[151AND8TH]

lol...i had to do that very dilution in my lab today...

Funny this was for a research lab also? I needed to do the dilution yesterday -- and late yesterday it was done....

thanks guys

 

[thefifthbeatle]

although it wasnt DNA (it was Na+ stock solution) I had to do the same dilution in advanced analytical chemistry!! See bio and chem overlap!!!

 

[smarbut]

he needs to end up with 1 ml

says who? I didn't get that from the first post. maybe I missed something.

 

[thefifthbeatle]

says who? I didn't get that from the first post. maybe I missed something.

read the third one..the first looked like densities, so i asked him to re type it