Gel Electrophoresis separates proteins based on size and charge?

[rak173]

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Or by size only since SDS gives negative charge to all proteins, so basically the only thing that matters is the size. I know that DNA is separated based on size and charge.

 

[ncide]

Polyacrylamide gel electrophoresis, called "Native PAGE" or "Nondenaturing PAGE" when SDS is not added, conserves the charge of the proteins and separates them according to their molecular weight-charge ratio. When SDS is added, it linearizes the proteins (destroys their tertiary structure) and coats them with the negatively charged molecule to offset the charge of their individual R groups. This is called SDS-PAGE. This means that SDS-PAGE separates according to their molecular weight. The intention of denaturing is to remove their 3-dimensional shapes that could slow down their progression through the gel (imagine you are comparing a linear protein and a ring protein with a higher surface area) and in this way standardize the proteins' solely on their molecular weight.

Hope this helps!

 

[rak173]

Polyacrylamide gel electrophoresis, called "Native PAGE" or "Nondenaturing PAGE" when SDS is not added, conserves the charge of the proteins and separates them according to their molecular weight-charge ratio. When SDS is added, it linearizes the proteins (destroys their tertiary structure) and coats them with the negatively charged molecule to offset the charge of their individual R groups. This is called SDS-PAGE. This means that SDS-PAGE separates according to their molecular weight. The intention of denaturing is to remove their 3-dimensional shapes that could slow down their progression through the gel (imagine you are comparing a linear protein and a ring protein with a higher surface area) and in this way standardize the proteins' solely on their molecular weight.

Hope this helps!

So SDS-PAGE separates based on Weight only and PAGE alone separates on Charge and Weight both?

 

[ncide]

Yes.