I agree with the previous post, verify with the school. I applied to UW Seattle and UM Baltimore and the average GPA they take is a little higher than 3.1 so it might hurt, but at the same time it might not.
I know that UW Seattle look at higher level courses more than general courses, so if you got an A in gen bio but this D+ they might consider this more. When they calculated my prereq GPA they used my higher level courses. I believe their interview is open file so they might ask you about it.
Good luck!