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- May 17, 2013
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I understand that Gram-positive bacteria absorb the stain well because of their thick peptidoglycan cell wall and Gram-negative don't because of their thin cell wall. EK Bio says that the Gram-positive cells appear purple and the Gram-negative cells appear pink. However, I just did a passage in TBR Bio (Ch. 6, passage 11) that outlines the gram-staining procedure. One of the questions asks which color each type of cell will be at the end. According to TBR, Gram-positive cells will be blue, and Gram-negative cells will be red. Now, even if you had read EK before doing this passage, you could still figure out the correct answer from the procedure they give you. But which of these is correct? Or are they supposed to be the same thing?