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Help with Gen Chem

Discussion in 'Pre-Medical - MD' started by premedmijo, Sep 29, 2000.

  1. premedmijo

    premedmijo Senior Member
    10+ Year Member

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    Can some one help me with a question from my Lab? Here it is:
    Suppose two cations have the same Rf value. How might you resolve their presence in a mixture using paper chromatography?

    Mucho Gracias in advanced!
     
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  3. If you're using paper chromatography, you can vary the solvent. Let's say one particular ion has a high solubility in acetone or something, then you could use acetone to keep that ion in solution, and it will travel farther than say, an ion that doesn't "like" acetone. Using different solvents, you can somewhat resolve different ions. The BEST way to do this is by using ion-exchange chromatography. Where you have polymer beads in a column and their charged ends are outward on the spherical(or nearly spherical) beads. You control the charge with pH and the cations bind/unbind based on size/charge density. It works best for transition metal elements. But you can resolve main group elements using this technique as well. Hope this helps.

    Imtiaz
     
  4. premedmijo

    premedmijo Senior Member
    10+ Year Member

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    Thanks Imtiaz
     

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