I took it without Orgo 2 my first time and then took it again after taking orgo 2 and while I'm sure people will tell you "it'll be fine" I'd say:
1)At least for me, I felt the orgo was much easier 2nd time around, I guess because orgo 2 was fresh on my mind and it made sense.
2)It depends on what your orgo 1 class teaches; for example if they leave some concept to be taught in orgo 2 that's part of the tested material then you may have a harder time than if your school's Orgo program addresses all the MCAT material.
Hope that helps even though it's a sort of ambiguous answer. My thought though it's probably not what you want to hear, is take it after Orgo 2. Orgo 2 really helped solidify the concepts for me and made the Orgo on the exam easier. Best of luck!