Mitochondrial Isolation; Anyone done it?

This forum made possible through the generous support of SDN members, donors, and sponsors. Thank you.

MSTPbound

student
Moderator Emeritus
10+ Year Member
Joined
Oct 1, 2006
Messages
520
Reaction score
0

Members don't see this ad.
Just wondering if anyone has (successfully) extracted mitochondria - if so, can you recommend a protocol? Actually, I am able to get them at the interphase of two buffers, but I'm having trouble figuring out the best way to get them out without pulling too much buffer (used a 27G needle, but still pulled too much buffer).

Thanks.

-MSTPbound
 

MSTPbound

student
Moderator Emeritus
10+ Year Member
Joined
Oct 1, 2006
Messages
520
Reaction score
0
Ok... I guess that means no. I finally figured it out; just had to break a 1-mL pipet in half and play with it a little bit.
 

stochastic

Member
10+ Year Member
5+ Year Member
Joined
Mar 31, 2005
Messages
68
Reaction score
0
I didn't check these links, but I have had success purifying them with Dynal M-500 magnetic beads conjugated to anti-porin antibodies.
 
Top