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Mitochondrial Isolation; Anyone done it?

Discussion in 'Physician Scientists' started by MSTPbound, Feb 27, 2007.

  1. MSTPbound

    MSTPbound student
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    Just wondering if anyone has (successfully) extracted mitochondria - if so, can you recommend a protocol? Actually, I am able to get them at the interphase of two buffers, but I'm having trouble figuring out the best way to get them out without pulling too much buffer (used a 27G needle, but still pulled too much buffer).

    Thanks.

    -MSTPbound
     
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  3. MSTPbound

    MSTPbound student
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    Ok... I guess that means no. I finally figured it out; just had to break a 1-mL pipet in half and play with it a little bit.
     
  4. stochastic

    stochastic Member
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    I didn't check these links, but I have had success purifying them with Dynal M-500 magnetic beads conjugated to anti-porin antibodies.
     

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