Dismiss Notice
SDN members see fewer ads and full resolution images. Join our non-profit community!

Mitochondrial Isolation; Anyone done it?

Discussion in 'Physician Scientists' started by MSTPbound, Feb 27, 2007.

  1. MSTPbound

    MSTPbound student
    Moderator Emeritus

    Joined:
    Oct 1, 2006
    Messages:
    520
    Likes Received:
    0
    Status:
    Post Doc
    Just wondering if anyone has (successfully) extracted mitochondria - if so, can you recommend a protocol? Actually, I am able to get them at the interphase of two buffers, but I'm having trouble figuring out the best way to get them out without pulling too much buffer (used a 27G needle, but still pulled too much buffer).

    Thanks.

    -MSTPbound
     
  2. Thread continues after this sponsor message. SDN Members do not see this ad.

  3. MSTPbound

    MSTPbound student
    Moderator Emeritus

    Joined:
    Oct 1, 2006
    Messages:
    520
    Likes Received:
    0
    Status:
    Post Doc
    Ok... I guess that means no. I finally figured it out; just had to break a 1-mL pipet in half and play with it a little bit.
     
  4. stochastic

    stochastic Member

    Joined:
    Mar 31, 2005
    Messages:
    68
    Likes Received:
    0
    Status:
    Medical Student
    I didn't check these links, but I have had success purifying them with Dynal M-500 magnetic beads conjugated to anti-porin antibodies.
     

Share This Page