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MSTPbound

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Just wondering if anyone has (successfully) extracted mitochondria - if so, can you recommend a protocol? Actually, I am able to get them at the interphase of two buffers, but I'm having trouble figuring out the best way to get them out without pulling too much buffer (used a 27G needle, but still pulled too much buffer).

Thanks.

-MSTPbound
 

MSTPbound

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Ok... I guess that means no. I finally figured it out; just had to break a 1-mL pipet in half and play with it a little bit.
 

stochastic

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I didn't check these links, but I have had success purifying them with Dynal M-500 magnetic beads conjugated to anti-porin antibodies.
 
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