So, Will the process go in this order?: reverse transcriptase to turn RNA into cDNA, then do PCR (why would u do PCR here?), then digest the cDNA to see if it has the specific fragment that codes for a certain protein, then another PCR (using specific primers) to obtain the specific fragment, then a miniprep to obtain plasmid from bacteria, then another digestion to add the fragment to the bacteria for expression. Does that look right?
Thanks
Thanks