Molecular Biology Question Please Help

[letaps]

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So, Will the process go in this order?: reverse transcriptase to turn RNA into cDNA, then do PCR (why would u do PCR here?), then digest the cDNA to see if it has the specific fragment that codes for a certain protein, then another PCR (using specific primers) to obtain the specific fragment, then a miniprep to obtain plasmid from bacteria, then another digestion to add the fragment to the bacteria for expression. Does that look right?

Thanks

 

[Phlame217]

gah double postin! I explained this in previous posts