Please confirm these--bio statements

[navneetdh]

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Bacteria (and prokaryotes??) do not contain Introns. This is why they produce proteins faster because they dont have to do RNA processing.

I read this statement somewhere in Princeton.

Again in cliffs they state that when do recombinant DNA--we need to remove the introns and therefore use processed m-RNA to make DNA via reverse transcriptase because the bacteria will prevent transcription if introns are present.

 

[lazybummy]

I'm not sure if that's the reason why it is faster.. from my understanding it is faster because everything is done.. (transcription, translation) in the same general location.

 

[BiomajorPreDent]

I'm not sure if that's the reason why it is faster.. from my understanding it is faster because everything is done.. (transcription, translation) in the same general location.

+1 That was exactly what I was going to say to you.

 

[Extirpator]

There are a variety of reasons why it's faster really. That's one of them... and what the others have said as well... and others. Although I'm not sure which would contribute the most to speed (I doubt it matters though).

 

[prsndwg]

I dont know about their lack of introns.. but they dnt have nucleus.. so the whole thing happens in the cytoplasm..

 

[UCB05]

As for the second statement, that too valid. Since prokaryotes lack the cellular machinery to excise introns, the recombinant DNA must be devoid of these sequences. Processed eukaryotic mRNA has already had these intron sequences excised, leaving just the ready-to-translate genomic protein sequence of interest. A reverse DNA transcript of that mRNA is made, then inserted into the prokaryote vector. This is concept behind a cDNA library.

 

[navneetdh]

I'm not sure if that's the reason why it is faster.. from my understanding it is faster because everything is done.. (transcription, translation) in the same general location.

yes that too is a reason they are faster.


SO no one is sure about the absence of introns?? SO can i take it that some prokaryotes do have introns?

 

[marscole]

Bacteria (and prokaryotes??) do not contain Introns. This is why they produce proteins faster because they dont have to do RNA processing.
Yes... prok including bacteria do not have introns. faster... compare to euk. protein expressions because euk. have series of transcription factors more than prok. in prok.

Again in cliffs they state that when do recombinant DNA--we need to remove the introns and therefore use processed m-RNA to make DNA via reverse transcriptase because the bacteria will prevent transcription if introns are present.
in reverse transcription (RT), there is no intron exits. post-transcriptional modification happens inside nucleus. in general RT procedures, mature mRNA is isolated RT it and the product is cDNA w/o any introns. they are all exons. i believe if you introduce any pre-mature RNA with exons and introns to the bacteria, and they just can't translate it since it's different information.

 

[navneetdh]

Thanks all 🙂

 

[dentrilla]

Bacteria (and prokaryotes??) do not contain Introns. This is why they produce proteins faster because they dont have to do RNA processing.
Yes... prok including bacteria do not have introns. faster... compare to euk. protein expressions because euk. have series of transcription factors more than prok. in prok.

Again in cliffs they state that when do recombinant DNA--we need to remove the introns and therefore use processed m-RNA to make DNA via reverse transcriptase because the bacteria will prevent transcription if introns are present.
in reverse transcription (RT), there is no intron exits. post-transcriptional modification happens inside nucleus. in general RT procedures, mature mRNA is isolated RT it and the product is cDNA w/o any introns. they are all exons. i believe if you introduce any pre-mature RNA with exons and introns to the bacteria, and they just can't translate it since it's different information.

yupyuppppppppp, was waiting for some1 to talk about cDNA 🙂

 

[marscole]

I think when people start to talk about cDNA (cloned DNA or complementary DNA), that is the product of reverse transcriptase (RT).😉

 

[dentrilla]

yah exactly, cDNA is a gene without introns, it gives us the ability to put genes into bacteria