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Hi,
I am an undergraduate researcher at University of Georgia. I am currently assigned the task of taking mouse embryonic fibroblast (MEF) and coming up with a differentiation medium to culture the cells into various tissue. I would like to differentiate the MEF into cardiomyocytes or neuronal tissue. I have found two research articles which I will link below that have done this successfully, however, I am but a meek person and I need help understanding the methods and process of doing this.
The instructor I work with is wanting me to do this project independently. So I have to figure out what kind of media I need to use, all the components and growth factors that must be added. I must time the experiment right, use appropriate conditions, and see it through ( MEF ---> tissue).
Would anybody knowledgable in this area please give me some help or advice? I sure would appreciate it.
http://www.cell.com/action/showMethods?pii=S0092-8674(10)00771-3 (Cardiomyocytes)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829121/ (Neuronal cells)
I am an undergraduate researcher at University of Georgia. I am currently assigned the task of taking mouse embryonic fibroblast (MEF) and coming up with a differentiation medium to culture the cells into various tissue. I would like to differentiate the MEF into cardiomyocytes or neuronal tissue. I have found two research articles which I will link below that have done this successfully, however, I am but a meek person and I need help understanding the methods and process of doing this.
The instructor I work with is wanting me to do this project independently. So I have to figure out what kind of media I need to use, all the components and growth factors that must be added. I must time the experiment right, use appropriate conditions, and see it through ( MEF ---> tissue).
Would anybody knowledgable in this area please give me some help or advice? I sure would appreciate it.
http://www.cell.com/action/showMethods?pii=S0092-8674(10)00771-3 (Cardiomyocytes)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829121/ (Neuronal cells)