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For the following restriction endonucleases, calculate the average distance between
restriction sites in an organism whose DNA has a random sequence and equal
proportions of all four nucleotides (25% each of A, T, C and G). The symbol R means
any purine (A or G), Y means any pyrimidine (T or C), and N stands for any nucleotide
(A, T, C or G).
Sau I
5' CCTNAGG 3'
RsaI
5' GTAC 3'
CviII
5'-RGCY-3'
I know how to do the RsaI and I got 1 out of 256 ie; 1/(4^4)
how do I do Sau I when there is the letter N or Y/R?
would the probability be 1 or 100% for N? and 50% for y/r?
thanks.
restriction sites in an organism whose DNA has a random sequence and equal
proportions of all four nucleotides (25% each of A, T, C and G). The symbol R means
any purine (A or G), Y means any pyrimidine (T or C), and N stands for any nucleotide
(A, T, C or G).
Sau I
5' CCTNAGG 3'
RsaI
5' GTAC 3'
CviII
5'-RGCY-3'
I know how to do the RsaI and I got 1 out of 256 ie; 1/(4^4)
how do I do Sau I when there is the letter N or Y/R?
would the probability be 1 or 100% for N? and 50% for y/r?
thanks.