Section Bank Bio #93

[wheatthinners]

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[theonlytycrane]

With II, we can tell that the siRNA won't affect other proteins which means the results must be from either FASN and/or mtKAS silencing. III helps to ensure that the siRNA is specific for FASN and mtKAS through the mechanism that it is believed to be working through.

 

[aldol16]

From my understanding, even if other random siRNA's DID interfere with FASN or mtKAS, they were never present in any of the trials to begin with. The results would still be the same, since this is a controlled environment and no other siRNA's were present. I get that if you wanted to extrapolate the data to in vivo, then III would be important, but in this experiment alone why would III be necessary?

You missed the point. The idea is not that other random siRNAs would affect these proteins. If that were the idea, they would have to test an almost-infinite number of siRNAs. The idea is that maybe all siRNAs would bind to certain genes and knock them down. In that case, you could be getting knockdown of a lot of various genes that could all cause the observed effect. To ascertain that it's not the siRNAs themselves but rather the siRNAs that are specific to these two genes that causes the effect, you must do III.

 

[wheatthinners]

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