Anyone out there with an SV40 IHC set-up?

[deschutes]

---

Members don't see this ad.

The context is transplant kidney biopsies and the sticky question of acute rejection vs. polyoma virus. Anyone here working with SV40 IHC?

 

[LADoc00]

The context is transplant kidney biopsies and the sticky question of acute rejection vs. polyoma virus. Anyone here working with SV40 IHC?

I have never seen a SV40 IHC ab in clinical use. Regardless, I would suggest looking for an ISH than IHC solution to this.

 

[yaah]

They've tried it here (I think) mostly on urine cytologies (thin preps, not cell blocks) as an antibody but problems have been lots of non-specific and false positive staining. The ones I saw had basically every cell staining. So I don't know how useful it is. Also don't know if they have done it on fixed paraffin tissue.

 

[alois]

There are extremely robust antibodies to large T antigen which is highly conserved across the polyoma viruses. These are used by many neuropathology centers for confirmation of the diagnosis of progressive multifocal leukoencephalopathy (PML), since the JC virus large T is recognized by the same antibodies.

So, I would find the nearest neuropathology group and ask them how they work up probable PML cases. That should get you to someone who will help.

 

[yaah]

I thought a lot of those places did FISH for that purpose...

 

[DrBloodmoney]

Yeah, we have one up and running. We recently began using it clinically, after a long time being refined / validated during one resident's BK virus research project.

 

[deschutes]

DrBloodmoney, is this going to be a paper any time soon? 🙂

 

[DrBloodmoney]

DrBloodmoney, is this going to be a paper any time soon? 🙂

I think it is in editorial limbo / hell just like my C4d paper. Damn renal journals 😡

 

[DarksideAllstar]

I think we have one here.

 

[deschutes]

The reason I got involved is that the county hospital apparently has been trying to bring it on board, but it always got buried under other priorities. Also there was something about a tech "not being able to find a place that sold the antibody".

So I found this:
http://www.scbt.com/table-sv40_t_ag.html

And then I was at a loss, because how do you pick one?

 

[Dr007NC]

we have been using SV40 immunostains on renal transplants and for opportunistic infection of other organs in immunosuppressed patients for many years now at Duke. It works fine on paraffin embedded tissues.

 

[pathstudent]

it is routinely used at my institution, even on cytology specimens, and it works outstandingly. I can't believe it isn't "standard of care" nationally at least at transplant academic institutions. It's as good at a CMV stain or Herpes stain.

 

[sohsie]

We have it here. It seems to work fine

 

[deschutes]

Anyone want to do me a favour and ask a tech for the product name/catalog number??