Biochemistry

[Missserica]

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Hey guys, I posted this on the DAT forums but there are WAY more people on the Pre-dental forum, so hopefully someone can help me.


Enzyme X is a highly pigmented protein that imparts the characteristic color to certain blue-green algae. It also facilitates a reaction necessary to the survival of this species; we can follow the progress of this reaction by measuring the conversion of Substance X to Substance Y.

1. Given a pure preparation of these algae, and the required supplies and equipment, devise and outline an empirical procedure for purifying Enzyme X.

Photosynthesis is not involved in the answer..

Ok, any help would be mucho appreciated.

Thanks.

 

[fightingspirit]

Hey guys, I posted this on the DAT forums but there are WAY more people on the Pre-dental forum, so hopefully someone can help me.


Enzyme X is a highly pigmented protein that imparts the characteristic color to certain blue-green algae. It also facilitates a reaction necessary to the survival of this species; we can follow the progress of this reaction by measuring the conversion of Substance X to Substance Y.

1. Given a pure preparation of these algae, and the required supplies and equipment, devise and outline an empirical procedure for purifying Enzyme X.

Photosynthesis is not involved in the answer..

Ok, any help would be mucho appreciated.

Thanks.

well, well, well...

extract all proteins by repeated freezing and thawing cycles, then the vial (labeled 1) should contain protein precipitates and soluble proteins in solution. take out the supernatant and centrifuge it, then get rid of the supernatant and lebel the vial "2". then use some form of column chromatography to seperate all the proteins based on size. then label vials according ot size range. then take a bit of what's in each vial and add to it substance x (after having labeled the carbons of that substance off course). since you dont know what substance y is like, then you depend on the rate of substance x's depletion to detect the presence of the enzyme. record the negative change in concentration of x (through fluorescence change) per certain amount of time that is fixed regardless of what protein is being tested. once you identify the vial that has the mix of proteins that have the most enzyme X, then take a bit of that vial and run SDS-page electrophoresis. that would give you an idea about the size of the different proteins you have in there. the difference in their sizes should be your guide in chosing the suitable beads for the column chromatogpraphy. then run just that vial through the column and retest with substance x. voila!!!...

 

[djtiesto14]

Can you tell me which book this is from?