Buffer question

[Frank Hardy]

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For those of you who use it- is TAE (Tris acetate EDTA) for DNA gels pH'd to 8.0 or 8.5? Need to know ASAP. Thanks!

 

[Hard24Get]

8.0

For those of you who use it- is TAE (Tris acetate EDTA) for DNA gels pH'd to 8.0 or 8.5? Need to know ASAP. Thanks!

 

[hawkeey]

8.0

Usually 8.0 but you can prepare the buffer with slightly lower or higher pH by adding either acid or base.

 

[Frank Hardy]

Thanks.

I already made it with 8.0. I don't think it's critical.