hey all, i have a basic chem question.. im trying to add an inhibitor solution to my cell culture and i need some help preparing it. i want to prepare a solution of 100 microMolar. i have 1 mg of the powder and the volume in which i want to place my inhibitor solution is 2 mL. the MW of the inhibitor is 270 g/mole i know i have 3.7x10^-6 moles of the inhibitor. if i add the 1 mg of the inhibitor to 37 mL of water i will have the solution of 100 microMolar. however, what i am wondering is if i have 2 mL of media that i want to add my inhibitor to, at what concentration should i prepare the solution and how much inhibitor solution should i add... does this make sense? sorry, ive forgotten my basic chem and my brain is fried right now. Moderator note: SDN is not to be used as a homework help service. Please approach your professors, teaching assistants, and friends from class if you are in need of assistance.