mol bio question

Discussion in 'Step I' started by buddindoc, Dec 20, 2005.

  1. buddindoc

    buddindoc Senior Member
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    can some one explain this?

    IF THE GRAPH SHOWN BELOW REPRESENTS DNA CONTAINING NORMAL MIX OF BASES,IT CAN BE EXPECTED THAT THE GRAPH WILL SHIFT TO THE LEFT FOR DUPLEX DNA CONTAINING HIGH CONTENT OF

    A)A+C
    B)C+G
    C)A+T
    D)C+T
    E)A+G A=ADENINE,C=CYTOSINE,G=GUANINE,T=THYMINE

    Y AXIS=OPTICAL DENSITY
    X AXIS=TEMP



    E
    /--------
    / D
    / B
    A /
    -------- C
     
  2. buddindoc

    buddindoc Senior Member
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    PS I TRIED TO DO THE GRAPH
    DID NOT HAPPEN

    IT LOOKS LIKE A without the curves


    hope this helps :)
     
  3. buddindoc

    buddindoc Senior Member
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    god ...LOOKS LIKE A "S" WITHOUT THE CURVES
     
  4. SoCuteMD

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    Ummm...would probably help if you told us what was on the X and Y axes.
     
  5. G0S2

    G0S2 SDN Angel
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    I believe A+T is the weakest base pair thermodynamically, therefore, the curve will shift to the left and a molecule of DNA made from a majority of A+T will denature into 2 strands (increasing optical activity) at a lower temp than another piece of DNA (of equal length) with G+C's.
     
  6. buddindoc

    buddindoc Senior Member
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    thanks that is the answer and thanks for xplaining it to me
    appreciate it
     

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