Random non-MCAT and only peripherally related to the MCAT thread - Part 3

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Nikki2002

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3rd time is a charm! 🙂 Welcome to the best thread on SDN: The Random Non-MCAT thread--PART 3!

This is a place to chill and talk about anything besides the MCAT. 😎



And remember......

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MollyMalone said:
Gah. Troubleshooting sucks 95% of the time.

The 5% of the time you have a crazy idea that turns out to be good, it's awesome.
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This trouble shooting isn't going to get us far I fear. Apparently they had a mycoplasm problem about a month ago and cleaned the incubator. I really don't know. But what I'm doing now probably isn'tgoing to pinpoint much at this point. But hey, I'm trying and the PI is the one that gave methe instructions 😉

actually i just checkedand it was cleaned for the same problem back on 6/19
 
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mshheaddoc said:
This trouble shooting isn't going to get us far I fear. Apparently they had a mycoplasm problem about a month ago and cleaned the incubator. I really don't know. But what I'm doing now probably isn'tgoing to pinpoint much at this point. But hey, I'm trying and the PI is the one that gave methe instructions 😉

actually i just checkedand it was cleaned for the same problem back on 6/19
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Grrr. You are definitely in the 95%. I feel for you. And I am ridiculously happy that I decided not to work in my old lab this summer. 😀
 
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mshheaddoc said:
Oh well (about the cells). We'll have to start fresh on monday. THe lab tech is out today so I'm doing this all by myself. I could start the new cells but we have to make fresh media if its the media that's tainted. SOOOOOO I'm testing the media but if its the incubator that is contaminated, that's not going to help us much now is it? 😎
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If they just became contaminated recently ( last night or so) try diluting the cell culture into many, many dilutions and see if you can start a clean one. But it may be too infected at this point, yeast sucks. 👎
 
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DRKUBA said:
If they just became contaminated recently ( last night or so) try diluting the cell culture into many, many dilutions and see if you can start a clean one. But it may be too infected at this point, yeast sucks. 👎
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Well I'm glad its not a yeast at least and that is something I had thought of but the PI just said to change the media on the flasks and go with my experiment (chamber slides). So its just cells treated with hormones. There wasn't alot of contaimination but I did want to change flasks and he said just change media and let it be. blast.
 
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mshheaddoc said:
That was the original thought but it got nixed. 🙁 I really wanted it in vegas too.
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Didn't the majority want Vegas? Why did it get switched? 🙁

I've been looking for an excuse to go back to Vegas. I've been their twice (my wedding and with a bunch of girlfriends) and it is so much fun there.
 
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mshheaddoc said:
My cells are contaminated. Today just isn't my day. ALL of our cells are contaminated somehow but I still have to do this 6 hr experiment 😎 Kinda pointless at this point. Mcyoplasm is bad. I wish it had been a fungus then I def wouldn't have to do it. 🙁
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Darn that sucks. I know how that feels.
 
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It was vegas but chicago wasn't far behind. Some people mentioned the weather, others mentioned how expensive vegas would be. I also thought about how alot of stuff is 21+ in vegas so I didn't know if that would be an issue either. I still wish it would be in vegas but the way it sounds is that things are towards chitown. Maybe we'll convince vegas the year after 😉
 
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mshheaddoc said:
It was vegas but chicago wasn't far behind. Some people mentioned the weather, others mentioned how expensive vegas would be. I also thought about how alot of stuff is 21+ in vegas so I didn't know if that would be an issue either. I still wish it would be in vegas but the way it sounds is that things are towards chitown. Maybe we'll convince vegas the year after 😉
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Mushy, we had this debate before. I think the population density within the driving radius of Chitown is greater than that of LV. For a first time event, not many people will drop serious coin to get there until it is a proven success.
 
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btw... they're 22.49 at sports authority. i'm hoping they didn't have the x2 for the same price... cause i'm going back to get them
 
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I triaged 23 patients this morning in 2 hours!! 😱 I am SO INSANELY TIRED and I can't switch back to thinking in English. I've started speaking in Spanish twice to the nurse who just cracks up each time. :laugh:
 
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sometimes its easy to forget to eat. when i get too involved in something i will remembe when someone tells me to do it.

heh, reminds me of back when i was volunteering... i wouldn't get up from my seat until after 4pm... when all the pastries in au bon pan went half off!
 
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BrettBatchelor said:
Mushy, we had this debate before. I think the population density within the driving radius of Chitown is greater than that of LV. For a first time event, not many people will drop serious coin to get there until it is a proven success.
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I see you're point but that wasn't the main reason why vegas got nixed. Cost seemed to be an issue, but as I said, I'm not sure what is going on with it.
 
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