For this one, I chose A because I thought PCR was a common technique used generally in biotech procedures to amplify a DNA sample so that you have enough for whatever type of analysis you are doing? I see why B is correct though, since IEF is based on the isoelectric point of proteins.



5+ Year Member
Jun 27, 2014
Medical Student (Accepted)
Hey! I know this is an older post, but thought I'd give it a try in case others were wondering/want to add their insight.

You have to read the question carefully. What MUST be included to visualize the bands in isoelectric focusing? Although PCR is an important step for amplification, if you don't have a proper pH gradient, we can assume that the bands might not even separate. Thus, we'd have no visualization.
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