To be fair, when I first applied I called a lower-tier MD school I had silence from in February. The portal said I was complete but the secretary told me the secondary payment didn't reach them for whatever reason. I made the payment and got an interview a week later. It was in March and I was waitlisted. Sometimes things happen and its still something I kick myself over.
When you call you're going to get an administrative assistant. Maybe they'll be nice or maybe they'll be cranky. It seems highly unlikely they'll remember you 24 hour later, or put a gold star or frowny face sticker on your file for asking. Be nice and don't call back in 2 weeks.