- Joined
- Jan 28, 2008
- Messages
- 82
- Reaction score
- 4
Alright so I was just in the lab about to finish up splitting some cells and then call it a day. I took the FBS out of the fridge and without even thinking, for some reason my head was somewhere else, I opened the bottle outside of the sterile hood. It is a large/expensive bottle and I know that I probably contaminated it. I just closed it up and put it back into the fridge, before I tell my PI is there anyway to decontaminate an entire bottle? Like maybe pour some of it out and hope the rest isn't contaminated?
Could I just put it through a vacuum sterilization? I dunno, are there any options?
Could I just put it through a vacuum sterilization? I dunno, are there any options?