can u tell us what ur daily routine consist of as a phd candidate?
Not sure if you're talking to me or gella, but I'll give you my take.
Research Phase
Head into lab @ 8:00
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Pour 3-4 gels, prep samples for running, pour stack, degas
Do normal tissue culture buffer x-changes (move confluent cells, throw away what's not needed ext.)
Start gels
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Go to lunch
Prep LC columns, prep LC samples
Stain gels and Transfer for westerns
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Finish LC run
Block membrane
Go home.
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@ Home... write everything in lab book (hope you didn't forget anything)
Go back into get laptop
Rinse and repeat.
Took about 300 gels, 100 westerns to get my results.
1/month, do animal studies. Collect tissue samples for above analysis.
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While doing this, you typically have 3-4 classes to teach, Journal clubs, and your own classes (1st 2 years)
Writing Phase:
Write Write Write. Question all your results. Convince yourself that your results are good. Throw away bad data (yup), and make an argument. You have to convince yourself that your DONE. - No: "One more experiment"
Grant Writing: (Required for academia if you ever want funding and tenure, assuming your university is still offering it)
I'll get back to you. But so far, 10x harder than anything I've done so far